What are MX Cre mice?

Conditional knockout mice are commonly used to study the function of specific genes in hematopoiesis. Different promoters that drive Cre expression have been utilized, with the interferon-inducible Mx1-Cre still being the most commonly used “deleter strain” in experimental hematology.

How does Mx1 Cre work?

The Cre recombinase is under the control of the Mx1 promoter, which can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA (such as poly I:C); enabling the “knockout” at any time during development in progeny of a floxed mutant mated to …

What is CMV Cre?

Also Known As:CMV-Cre These CMV-Cre mice express a transgene containing cre under the transcriptional control of a human cytomegalovirus minimal promoter.

What is a conditional knockout mouse?

A conditional Knockout mouse defines an animal model in which a gene of interest can be: Inactivated in specific cell types in a certain tissue; other cell types and tissues exhibit an unmodified, functional gene expression.

Is the mx1-cre transgene silent in healthy mice?

This transgene expresses Cre recombinase under the control of an inducible Mx1 promoter, which is silent in healthy mice, and active in the liver and in lymphocytes after induction with IFN or pI-pC. The construct also contains a 2.1 kb fragment from the human growth hormone gene. Kuhn R; Schwenk F; Aguet M; Rajewsky K. 1995.

When to use the MX-Cre mouse strain in a publication?

When using the Mx-Cre mouse strain in a publication, please cite the originating article (s) and include JAX stock #003556 in your Materials and Methods section. Live mice available in varying quantities. Ask Customer Service for details. Terms are granted by individual review and stated on the customer invoice (s) and account statement.

Is the Cre recombinase under control of the MX1 promoter?

The Cre recombinase is under the control of the Mx1 promoter, which can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA (such as poly I:C); enabling the “knockout” at any time during development in progeny of a floxed mutant mated to this transgenic.

Which is the best CRE strain to use?

This has led to the majority of available Cre drivers, in which expression is under the control of promoter and/or regulatory sequences of individual genes such as transcription factors (e.g., Emx, Cux2, Ascl1, Dlx1) or those related to specific neurotransmitters or neuropeptides (e.g., Chat, Pomc, Th, Dbh ).