What does n Ethyl maleimide do?

Thermo Scientific Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.

What does NEM inhibit?

N-ethylmaleimide (NEM) inhibits the transport of phosphate in mitochondria but is without effect on permeation of other metabolities.

What is nem used for?

NEM offers seamless ability to interface between public and private blockchains. It enables easy transfer of any digital asset – like tokens, contract, or files – from a privately held internal enterprise network, routed via a public blockchain, and finally to another business’s private network.

Does NEM dissolve in water?

NEM gives a clear solution in ethanol at 50 mg/ml. NEM dissolves in water (>50 mg in 4 ml); however, aqueous solutions are unstable. The rate of hydrolysis is pseudo-first order and significantly dependent on pH.

How do you prepare n Ethylmaleimide?

Prepare 100-200mM of NEM in ultrapure water. Add a minimum of a 10-fold molar excess of NEM to sulfhydryl groups to be blocked. Alternatively, add an equal mass amount of NEM to the protein (e.g., add 2mg of NEM to 1mL of 2mg/mL protein).

What is NEM in solar?

Net metering (also known as net energy metering or NEM) is the foundational solar policy and incentive on which the entire residential solar industry is built. Simply put, net metering is a way of using the electric grid to store the energy produced by your solar panel system for use at a later time.

How do you dissolve NEM?

Dissolve 25 g NEM in ethanol and adjust volume to 100 ml. Store in aliquots at -20°C. NEM inhibits thiol proteases and prevents nonspecific disulfide exchange. Pepstatin A (0.2 mg/ml).

Is NEM cell permeable?

NEM a cell permeable, well known cysteine protease inhibitor. Most of the literature suggests it is used as an inhibitor during cell lysis for immunoprecipitation and western blotting.

What is the compound NEM?

N-Ethylmaleimide (NEM) is an organic compound that is derived from maleic acid. It contains the amide functional group, but more importantly it is an alkene that is reactive toward thiols and is commonly used to modify cysteine residues in proteins and peptides.

What is a free thiol?

Free Thiol Assay Kit (Fluorometric) (ab112158) provides an ultrasensitive fluorometric assay to quantitate thiol content that exists in small molecules such as free cysteine, glutathione, and cysteine residues. The assay can detect as little as 1 picomole of cysteine or GSH in a 100 µL assay volume (10 nM).

Which is the N-ethyl derivative of maleimide?

N-ethylmaleimide is a member of the class of maleimides that is the N-ethyl derivative of maleimide. It has a role as an EC 2.1.1.122 [(S)-tetrahydroprotoberberine N-methyltransferase] inhibitor, an EC 2.7.1.1 (hexokinase) inhibitor and an EC 1.3.1.8 [acyl-CoA dehydrogenase (NADP(+))] inhibitor.

What is the pH of N-Ethylmaleimide ( NEM )?

Description. N-Ethylmaleimide (NEM) is an alkylating reagent that reacts with sulfhydryls. At pH 6.5-7.5, the maleimide reaction is specific for sulfhdryls; however, at pH values greater than 7.5, reactivity with amino groups occurs. Maleimide groups react with sulfhydryls by nucleophilic attack of the thiolate anion on one of the carbons…

Where can I find the N-ethylmaleimide data?

Nature Chemical Biology, 2006. 10.1038/nchembio813 http://www.nature.com/naturechemicalbiology The data from CAS Common Chemistry is provided under a CC-BY-NC 4.0 license, unless otherwise stated. Unless otherwise indicated, all text within NCI products is free of copyright and may be reused without our permission.

What are the properties of Pierce N-ethylmaleimide?

Description Thermo Scientific Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation. Features of N-Ethylmaleimide (NEM):