Can anti-GFP detect YFP?
Q: What GFP variants will this antibody recognize? A: Our anti-GFP antibody is a mouse monoclonal raised against the N-terminal of GFP and will recognize native and denatured GFP and its variants, including EGFP, YFP, EYFP, and CFP.
Does GFP antibody recognize EGFP?
GFP antibody (ab6556) is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.
What is the difference between YFP and GFP?
GFP stands for Green Fluorescent Protein, which was originally derived from the jellyfish Aequorea Victoria. YFP stands for Yellow Fluorescent Protein. GFP exhibits an excitation peak at 395nm wavelengths,s and its emission peak is 509nm wavelengths. YFP emission peak is 527nm and excitation peak is 515 nm.
Can YFP and GFP be used together?
Concurrent use of GFP and YFP could be highly advantageous in many cellular assays, particularly when using cells of transgenic mice whose cells express fluorescent proteins, because generating mice with alternative fluorescent proteins can take a lot of time and effort.
What is anti GFP?
Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin.
Does GFP antibody recognize mCherry?
In most cases GFP and mCherry antibodies do not cross react, but you will need to check for yourself especially in the manufacturer does not mention it. These two antibodies do not cross react with the other fluorescent protein.
What wavelength does YFP absorb?
In the Nikon YFP HYQ block, excitation is limited to a 20-nanometer band of wavelengths in the blue-green spectral region (490 to 510 nanometers), and is coupled to an emission filter transmitting yellowish-green fluorescence in the range of 520 to 550 nanometers.
Can I use CFP and GFP?
“” Using CFP and GFP together can be challenging, but it is feasible. The CFP emission curve overlaps significantly with the GFP curve. However there is no significant excitation of CFP by the blue laser, so you should be OK in this regard.
Why is GFP fluorescent?
GFP is a barrel shape with the fluorescent portion (the chromophore) made up of just three amino acids. When this chromophore absorbs blue light, it emits green fluorescence.
How big is GFP?
GFP is big GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel is necessary for its fluorescence and therefore GFP cannot be downsized by deleting residues.
What is GFP and RFP?
Green Fluorescent Protein (GFP) and Red Fluorescent Protein (RFP) are ~29 kDa fluorescent proteins used as versatile markers for visualization of protein localization, monitoring physiological processes, and for detecting transgenic or transient protein expression.
Are there any antibodies that are reactive against GFP?
This antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP. Recombinant full length protein corresponding to GFP. Pure GFP protein, or cells known to overexpress GFP can be used as a positive control in WB.
Is there an anti GFP antibody for Aequorea victoria?
Anti-GFP Antibodies Green fluorescent protein (GFP) is a 27 kDa monomeric protein, isolated from the jellyfish Aequorea Victoria that auto-catalytically forms a fluorescent pigment. GFP is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression.
What’s the difference between a GFP and a YFP?
The main difference between GFP and YFP is that GFP exhibits green color upon exposure to light ranges from blue to ultraviolet whereas YFP exhibits yellow color upon exposure to the same light. Furthermore, GFP is originally derived from the jellyfish, Aequorea Victoria while YFP is…
What’s the difference between GFP and YFP in a jellyfish?
GFP refers to a protein that glows green under fluorescent light and found naturally in the jellyfish, Aequorea Victoria, while YFP refers to a genetic mutant of green fluorescent protein (GFP).
https://www.youtube.com/watch?v=PT1K-09Ny28