What does PAS highlight in a renal biopsy?
This normal glomerulus is stained with PAS to highlight basement membranes of glomerular capillary loops and tubular epithelium. The capillary loops of this normal glomerulus are well-defined and thin. The endothelial cells are seen in capillary loops. The mesangial regions are of normal size.
How is a renal biopsy performed?
During a kidney biopsy, your doctor uses a needle to remove a small sample of kidney tissue for lab testing. The biopsy needle is inserted through your skin and is often directed using the guidance of an imaging device, such as ultrasound.
What are the three methods of microscopy applicable to renal biopsy glomerular diseases?
INDICATION FOR RENAL BIOPSY The left kidney is visible without other organs impeding the acquisition of the tissue. An adequate assessment of native renal biopsies includes light microscopy (LM), immunofluorescence microscopy (IF), and electron microscopy (EM).
How would you transport sample for kidney biopsy?
Use the 95kPa Specimen Transport Bag for the specimen containers with the requisition in the outside pocket of the bag. Place the double-bagged specimen into a box with adequate absorbent material in case of leakage.
What is PAS stain used for?
The periodic acid-Schiff (PAS) staining procedure is most commonly used in the histology laboratory to detect glycogen deposits in the liver when glycogen storage disease is suspected. Glycogen granules may also be visible in tumors of the bladder, kidney, ovary, pancreas, and lung.
What should you not do after a kidney biopsy?
After the test, you will be told to lie down on your back for several hours. After this, you should avoid strenuous activity for the next 2 to 3 days. It’s normal to feel some soreness in the area of the biopsy for 2 to 3 days. You may have a small amount of bleeding on the bandage after the test.
How long does a renal biopsy take?
The entire procedure, from start to finish, usually lasts about one hour. Sometimes the biopsy may take longer than an hour. Open kidney biopsy: Some patients should not have a percutaneous biopsy because they may have a history of bleeding problems.
What will a kidney biopsy reveal?
A kidney biopsy helps doctors identify the cause of kidney problems so they can treat the condition effectively. It can reveal scarring, inflammation (swelling), and protein deposits that cannot be identified with other tests, such as ultrasounds or blood and urine tests.
Does PAS stain mucin?
PAS (periodic acid-Schiff): Stains glycogen as well as mucins, but tissue can be pre-digested with diastase to remove glycogen. Stains neutral and acid-simple non-sulfated and acid-complex sulfated mucins.
How serious is a kidney biopsy?
The risks of kidney biopsy are very small, but they should be discussed with your healthcare professional. As in other medical and surgical procedures, certain complications may happen even though every effort is taken to prevent them. A blood transfusion may be needed if serious bleeding occurs.
When to use basement membrane staining for renal biopsies?
Kidney: recommended for routine evaluation of renal biopsies due to basement membrane staining; also useful to diagnose renal cell carcinoma (stains glycogen, removed by diastase)
How is the PAS stain used in liver biopsies?
A routine stain for liver and kidney biopsies. PRINCIPLE: The PAS stain is a histochemical reaction in that the periodic acid oxidizes the carbon to carbon bond forming aldehydes which react to the fuchsin-sulfurous acid which form the magenta color. CONTROL: For staining fungus; use a known positive such as those used for the GMS.
What is the principle of the PAS stain?
PRINCIPLE: The PAS stain is a histochemical reaction in that the periodic acid oxidizes the carbon to carbon bond forming aldehydes which react to the fuchsin-sulfurous acid which form the magenta color. CONTROL: For staining fungus; use a known positive such as those used for the GMS. Use skin, aorta or normal liver for positive PAS staining.
What should you know about a renal biopsy?
A further advantage of the PAS stain is that alterations of the vessels, particularly arterial hyalinosis and fibrinoid necrosis, are easy to detect. Immune deposits can best be visualized by protein stains ( Figure 1 C) such as the acid fuchsin–Orange G stain (SFOG).