What is xMAP Luminex?

Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to 500, depending on system design. The microspheres used in the Luminex system have different spectral addresses (color codes).

What is xMAP Technology?

xMAP® – The World’s Most Used Multiplexing Technology xMAP® Technology allows users to perform a wide range of protein- and nucleic acid-based multiplex assays, which can simultaneously detect up to 500 targets in a single run.

How does a Luminex assay work?

A Luminex assay is a type of immunoassay that precisely measures multiple analytes in one sample. Antibodies specific to a desired analyte are coupled to a unique bead region and are incubated with sample.

Where is Luminex based?

Austin, Texas
Luminex Corporation

Type Subsidiary
Founded 1995
Headquarters Austin, Texas, United States,
Products xMAP, xTAG, MAGPIX, Luminex 100/200, FLEXMAP 3D, NxTAG, ARIES, VERIGENE, Amnis Cellstream, Amnis FlowSight, Amnis ImageStreamX Mk II, Guava Muse, Guava easyCyte
Website www.luminexcorp.com

Is Luminex an Elisa?

Enzyme-linked immunosorbent assay (ELISA) has long been the standard for quantitative analysis of metabolic hormones and cytokines. LUMINEX multiplex bead array assays were developed as cost- and time-effective alternatives to ELISA, but they are the only cost- and time-effective if they provide informative data.

Is Luminex more sensitive than ELISA?

The ELISA accuracy is 96.5%. Thus, the two assays are comparable for specificity and accuracy, and the Luminex assay is more sensitive than the ELISA.

What is multiplex immunoassay?

Multiplex assays are a type of immunoassay capable of simultaneous measurement of multiple analytes. Multiplex assays are derived from the ELISA format, but instead of producing a single signal measurement, they produce multiple signal measurements.

Is there a trend toward the development of multiplexed immunoassay?

There is also an emerging trend toward the development of multiplexed IA that holds a tremendous potential in “in vitro” diagnostics and bioanalytical sciences [32] as exemplified by the two most widely used formats: multiplex bead-based Luminex assays by Thermo Fisher Scientific and multiplex IA by Meso Scale …

What is multiplexing in diagnostics?

Multiplexing is the ability to identify a broad spectrum of conditions in a single molecular diagnostic test procedure, as opposed to conducting discrete individual procedures.

How to create a custom Luminex xMAP assay builder?

Use the Bio-Plex Assay Builder to design custom Luminex xMAP assays from popular assay groups in four easy steps. Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to 500, depending on system design.

How many targets can a Luminex xMAP system detect?

Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to 500, depending on system design. The microspheres used in the Luminex system have different spectral addresses (color codes).

What kind of Technology is used in Luminex?

Luminex multiplexing technology consists of xMAP bead-based immunoassays that can be analyzed using two types of instruments: flow cytometry-based analyzers (left schematic) and LED-based analyzer (right schematic). LED-based detection is used in compact multiplexing instruments like the MAGPIX® system.

How many analytes can Luminex 100 / 200 system measure?

The system enables you to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small samples. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid assays, receptor-ligand assays, immunoassays, and enzymatic assays.