What is the purpose of Nile Red?
Description. Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells.
How does Nile Red stain work?
In the assay, Nile Red dye is added to the cell samples and the dye selectively stains lipid droplets within the cells. The dye is minimally fluorescent in solution in water so it is not necessary to wash the cells or remove the dye before analysis.
How do you use the red Nile?
We outline a five-step method to customize the Nile Red protocol to a specific strain: 1) Evaluate the strain’s suitability by checking for the presence of neutral lipid, 2) Select of the best excitation/emission wavelength, 3) Optimization of incubation time, stain carrier, dye concentration, and temperature, 4) …
Does nile red fluorescence?
In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment, it can be intensely fluorescent, with varying colours from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages).
Is Nile red soluble in water?
In more polar ones its fluorescence emission shows a dramatic, and potentially useful, shift to about 640 nm, but its quantum yield is significantly reduced. Further, Nile Red has a very poor solubility in aqueous media.
How do you dissolve Red Nile?
o Nile Red Stock Solution (1 mg/mL) – dissolve 25 mg of Nile Red (Invitrogen N-1142) in 25 mL of DMSO; Store at room temperature in the dark indefinitely.
Who runs Nile red?
Nigel Braun began posting his chemistry experiments to YouTube in 2014 under the name NileRed (after the lipophilic stain) with no intention of amassing such a large audience. But now, four years later, he has over 420,000 subscribers that tune in regularly to watch his latest experiment.
Is Nile Red hydrophobic?
Nile red is an uncharged hydrophobic molecule whose fluorescence is strongly influenced by the polarity of its environment.
What kind of fluorescent stain is Nile red?
Recently, Nile red, an uncharged phenoxazone dye (Fig. 1), has been introduced as a fluorescent stain for intracellular lipids (10-12).
How is Nile red used to detect lipid deposits?
Application of Nile red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections: comparison with oil red O Nile red is a phenoxazone dye that fluoresces intensely, and in varying color, in organic solvents and hydrophobic lipids. However, the fluorescence is fully quenched in water.
How is Nile red a polarity sensitive fluorescent probe?
It detects the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation.
How does Nile red phenoxazone dye work?
Nile red is a phenoxazone dye that fluoresces intensely, and in varying color, in organic solvents and hydrophobic lipids. However, the fluorescence is fully quenched in water. The dye acts, therefore, as a fluorescent hydrophobic probe.