What does nutrient agar contain?

Nutrient agar is a general purpose medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): 0.5% Peptone – this provides organic nitrogen. 0.3% beef extract/yeast extract – the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts.

What is Difco nutrient broth?

Difco™ Nutrient Broth, BD. Media for Microbiology Dehydrated Media for Microbiology. Nutrient Broth is used for the cultivation of many species of nonfastidious microorganisms. Beef extract provides carbohydrates, vitamins, nitrogen, and salts.

What is the composition of nutrient broth?


Ingredient 1 L 500 mL
beef extract 1 g 0.5 g
Yeast extract 2 g 1 g
Peptone 5 g 2.5 g
Sodium chloride (NaCl) 5 g 2.5 g

Is nutrient agar toxic?

Not a dangerous substance or mixture according to the Globaly Harmonised System (GHS). Inhalation May be harmful if inhaled. May cause respiratory tract irritation. Skin May be harmful if absorbed through skin.

What is the pH of nutrient broth?

Nutrient Broth, pH 6.9 without NaCl

Quality Control
Appearance Cream to yellow homogeneous free flowing powder
Reaction Reaction of 0.8% w/v aqueous solution at 25°C. pH : 6.9±0.2
pH 6.70-7.10
Cultural Response Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.

What is the difference between agar and broth media?

The only difference between broth and agar media is that broths do not contain an agar component. We use broth tubes primarily for specific assays, or (rarely) for bacteria that will not form colonies on a solid surface. Unlike preparation of agar plates, tubes are prepared with media already in the incubation vessel.

Is nutrient agar selective or differential?

1. Observe the growth and appearance of colonies on all plates. Notice that nutrient agar is neither a selective nor differential medium.

What happens if you eat agar?

When taken by mouth: Agar is POSSIBLY SAFE for most adults when taken with at least one 8-ounce glass of water. If it is not taken with enough water, agar can swell and block the esophagus or bowel.