What is Gr1 antibody?
Anti-Gr1 antibody (RB6-8C5) was widely used and considered to be effective in eliminating MDSC. Thomas Condamine et al determined that anti-Gr1 antibody eliminated about 95% of MDSCs in spleen and blood of TB mice; however, it raised the immature myeloid cell (IMC) levels in the BM.
What is Ly6G?
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow.
What is neutrophil depletion?
Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, these approaches are only partially effective, transient or lack specificity.
What is Ly6C a marker for?
Our data identify Ly6C as a marker of functionally discrete tissue macrophage subsets and support a model of selective recruitment of Ly6Chigh bone marrow monocytes to the kidney that dif- ferentiate into three populations of kidney macrophages, including a profibrotic Ly6Clow population.
What does Ly6C stand for?
Official Symbol Ly6c1provided by MGI Official Full Name lymphocyte antigen 6 complex, locus C1provided by MGI Primary source MGI:MGI:96882 See related Ensembl:ENSMUSG00000079018 Gene type protein coding RefSeq status VALIDATED Organism Mus musculus Lineage Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; …
Are neutrophils Ly6G+?
GFP identifies Ly6Chi monocytes, and Ly6G identifies neutrophils. Data are presented as the arithmetic mean ± SEM.
What is Ly6C high?
Monocytes expressing high levels of Ly6C (Ly6Chi monocytes) have proinflammatory and antimicrobial functions and express high levels of C-C che- mokine receptor 2 (CCR2) and low levels of CX3C chemo- kine receptor 1 (CX3CR1; CCR2hiCX3CR1low).
What cells are CD45+?
CD45 antigen (leukocyte common antigen), a unique and ubiquitous membrane glycoprotein with a molecular mass of about 200 kDa, is expressed on almost all hematopoietic cells except for mature erythrocytes.