Are Jurkat cells adherent or suspension?
Jurkat Clone E6-1 is a human T lymphoblastoid cell line derived from an acute T cell leukemia. The cells are suspension lymphoblasts. The karyotype is pseudodiploid human male cell line.
How do Jurkat cells grow?
1. Maintain Jurkat cells in RPMI with l-glutamine supplemented with 10% fetal bovine serum at 37°C, 5% CO2 in a humidified incubator. Grow cells to no more than 2 × 105 cells/mL and passage cells 2–3 times a week.
What kind of T cells are Jurkat cells?
Jurkat is an acute T cell leukemia cell line originally derived from the peripheral blood of a 14-year-old boy. The original Jurkat clone was designated JM*. The E6-1 clone, derived from JM, is now the Jurkat cell line used most frequently by researchers, but many other clones or derivatives are available.
How do you culture non-adherent cells?
Passaging Suspension Cultures This can be done by directly diluting the cells in the culture flask and continue expanding them, or by withdrawing a portion of the cells from the culture flask and diluting the remaining cells down to a seeding density appropriate for the cell line.
What are non-adherent cells?
Non-adherent cells Many cell types, in particular, many microorganisms, grow in solution and not attached to a surface. These cell types can be subcultured by simply taking a small volume of the parent culture and diluting it in fresh growth medium.
How big is a Jurkat cell?
Jurkat cells are an immortalized line of human T lymphocyte cells of highly spherical shape with the diameter ranging from 10 to 16 µm, first derived from the peripheral blood of a 14-year-old boy suffering from T cell leukemia [33]. Jurkat cells can be transfected and can produce interleukin-2 (IL-2) [34,35].
Why are they called Jurkat cells?
Cell Line Description Jurkat cell line was established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is called “JM” (JURKAT and JM are derived from the same patient and are sister clones).
What cells are adherent?
Adherent cells are cells which must be attached to a surface to grow. They are commonly used in laboratory environments. However, to produce biopharmaceuticals, the preference has been to use suspension cells, often Chinese hamster ovary (CHO) cells, in traditional stirred tank reactors.
How long does it take adherent cells to attach?
If you trypsinized the adherent cells then replate them probably only need a few minutes for some of them to attach. For both endothelial and sommoth muscle cells I have seen them attached withing 20 minutes whereas from liquid N2 it may take 2-4 hours.
How do you culture non adherent cells?
What are Jurkat cells and what are they used for?
Jurkat cells. Jump to navigation Jump to search. Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV.
How to make a subculturing Jurkat cell line?
Subculturing Jurkat Cell Line. Protocol. Transfer growing culture from T75 flask to a sterile 50 ml conical tube. Centrifuge culture at ≤ 1,500 rpm for 2-3 minutes at room temperature. Remove and discard 10 ml of old growth medium from above the cell pellet.
How to make non adherent cell ( Jurkat ) culture protocol?
Thaw a 1-ml aliquot of cells as quickly as possible in water bath at 37o cells to 9 ml warm media in a 15-ml conical tube. Mix gently. Centrifuge at 1,200 rpm for 5 minutes to pellet cells. Discard media and resuspend pellet gently in 10 ml warm medium. Divide cells into two T-25 flasks containing 5 ml warm media. Place in incubator.
What are the mutations in the Jurkat cell line?
Different derivatives of the Jurkat cell line that have been mutated to lack certain genes can now be obtained from cell culture banks. The JCaM1.6 cell line is deficient in Lck activity due to the deletion of part of the LCK gene ( exon 7) from the LCK transcript.